Live Single-Cell Mass Spectrometry for Single Cell Organelle Molecular Analysis
نویسندگان
چکیده
منابع مشابه
Live single-cell molecular analysis by video-mass spectrometry.
The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peak...
متن کاملLive single-cell mass spectrometry.
The history from bio-imaging to live single-cell mass spectrometry (MS) is herein reviewed. The limitation of the current bio-imaging method is probing only known molecules, and a method for finding new molecules is needed for cells which, however, show individual behaviors even in the same incubation dish. Single-cell MALDI-TOF/MS has been developed, but it can detect only molecules that can b...
متن کاملFluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism.
Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell...
متن کاملLive Single-Cell Plant Hormone Analysis by Video-Mass Spectrometry.
Studies have indicated that endogenous concentrations of plant hormones are regulated very locally within plants. To understand the mechanisms underlying hormone-mediated physiological processes, it is indispensable to know the exact hormone concentrations at cellular levels. In the present study, we established a system to determine levels of ABA and jasmonoyl-isoleucine (JA-Ile) from single c...
متن کاملDirect single-cell molecular analysis of plant tissues by video mass spectrometry.
Direct and quick molecular analysis of live plant single cells viewed under a video-microscope has been developed in their intact undamaged environment using nano-electrospray tip and mass spectrometers. This method has been successful in identifying specific molecules in plant live single-cell analysis by mass spectrometry, and opens the possibility to compare different cell types from differe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Mass Spectrometry Society of Japan
سال: 2020
ISSN: 1340-8097,1880-4225,1884-3271
DOI: 10.5702/massspec.s20-08